Goat Anti-Human IgG (H+L) HRP

Product Highlights

Cat No: SB-AB0104

Antibody Size: 2.0 mL (Concentration: 0.8 mg/m)

Application: WB ELISA, IHC

Reactivity: Human IgG

Host Species: Goat

Immunogen: Human IgG

Description

Overview
Antibody Name Goat Anti-Human IgG (H+L) HRP
Antibody SKU SB-AB0104
Antibody Size 2.0 mL (Concentration: 0.8 mg/m)
Application WB ELISA, IHC
Reactivity Human IgG
Host Species Goat
Immunogen Human IgG
Applications
Application WB, ELISA, IHC
Recommended Dilution
  • 1:500 – 1:5,000 for immunohisto/cytochemistry
  • 1:5,000 – 1:100,000 for ELISA and Western blotting with chromogenic substrates
  • 1:10,000 – 1:200,000 for Western blotting with ECL substrates
Immunogen Human IgG
Purification Method The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
Storage Buffer Store freeze-dried solid at 2-8°C. Rehydrate with 2.0 ml dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid
Isotype Horseradish peroxidase conjugated IgG
Stabilizer 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Extended Storage after Rehydration Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Antibody Specificity Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Note For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.

Data Sheet

COA

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